Fig. 6: The antifibrotic effect of orally administered miR2911 is abolished in Sidt1-knockout mice. | Cell Research

Fig. 6: The antifibrotic effect of orally administered miR2911 is abolished in Sidt1-knockout mice.

From: SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs

Fig. 6

a Diagram of miR2911 gavage feeding or intravenous injection treatment in a CCl4-induced liver fibrosis mouse model. b–d The miR2911 (b), TGF-β1 (c) and hydroxyproline (d) levels in livers of Sidt1+/+ or Sidt1−/− mice after gavage feeding (Sidt1+/+, n = 8; Sidt1−/−, n = 7) or intravenous injection (Sidt1+/+, n = 7; Sidt1−/−, n = 7) of miR2911. Two-way ANOVA with Sidak’s post hoc test. ns, not significant, ****P < 0.0001. e Representative images (upper panel; scale bar, 100 μm) and quantification (bottom panel; n = 6) of immunostaining for α-SMA and collagen I in Sidt1+/+ or Sidt1−/− liver tissue sections after gavage feeding or intravenous injection of miRNA. a.u., arbitrary unit. One-way ANOVA with Tukey’s post hoc test. ns, not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001. f Representative images (left panel; scale bar, 200 μm) and quantification (right panel; n = 6) of Sirius red staining in Sidt1+/+ or Sidt1−/− liver tissue sections after gavage feeding or intravenous injection of miRNA. One-way ANOVA with Tukey’s post hoc test. ns, not significant, ***P < 0.001, ****P < 0.0001.

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