Fig. 6: Knockdown of iGluRs decreased the excitability and conductivity in the cultured human iPSC-derived atrial cardiomyocyte monolayers. | Cell Research

Fig. 6: Knockdown of iGluRs decreased the excitability and conductivity in the cultured human iPSC-derived atrial cardiomyocyte monolayers.

From: Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes

Fig. 6

a Experimental protocol for the MEA analysis in cultured human iPSC-derived atrial cardiomyocyte monolayers. b, c Knockdown efficiency of GRIA3 (b) and GRIN1 (c) in the human iPSC-derived atrial cardiomyocytes at mRNA level. *P < 0.05, calculated by Student’s t-test, n = 3. d, e Knockdown efficiency of GRIA3 (d) and GRIN1 (e) in human iPSC-derived atrial cardiomyocytes at protein level. *P < 0.05, calculated by Student’s t-test, n = 3. f Typical heatmap of the excitatory firing rate in the cultured atrial cardiomyocyte monolayers with GRIA3 or GRIN1 knockdown. g Pooled data of firing rate from f. *P < 0.05, calculated by Student’s t-test, n = 5−7 per group. h Typical conduction heatmap of cultured atrial cardiomyocyte monolayers with GRIA3 or GRIN1 knockdown. i Pooled data of conduction velocity from h. *P < 0.05, calculated by Student’s t-test, n = 5−8 per group.

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