Fig. 9: Expression and functional role of the glutamatergic transmitter system in human atrial cardiomyocytes. | Cell Research

Fig. 9: Expression and functional role of the glutamatergic transmitter system in human atrial cardiomyocytes.

From: Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes

Fig. 9

a qPCR analysis of the core elements of the glutamatergic transmitter system in human atrial cardiomyocytes illustrating that GLS, GRIA3, GRIN1 and SLC1A3 were highly expressed in human atrial cardiomyocytes at mRNA level. b Western blot analysis of the core elements of the glutamatergic transmitter system illustrating that GLS, GRIA3, GRIN1 and EAAT1 are expressed in human atrial cardiomyocytes at protein level. c Immunofluorescence images of the core elements of the glutamatergic transmitter system in human atrial samples showing the spatial location of GLS, GRIA3, GRIN1, and EAAT1 in the human atrial cardiomyocytes. Scale bar, 50 μm. d Representative patch-clamp recordings of whole-cell currents elicited by the application of 1 mM glutamate in human atrial cardiomyocytes. The holding potential was set at −60 mV. e Pooled data of the glutamate-elicited currents induced by different concentrations of glutamate in isolated human atrial cardiomyocytes. n = 6.

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