Fig. 5: The heterogeneity of conventional and unconventional ILC2 subsets.
From: Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells
a UMAP visualization of Group 2 ILC with five subpopulations. Colors indicate cell types. b Expression of the indicated genes projected to UMAP of ILC2 sub-clusters. Colors indicate the scaled gene expression level. c Violin plots show the expression of featured genes in ILC2 sub-clusters. Colors represent cell clusters. d UMAP visualization of ILC2 sub-clusters described in a. Stage (left panel) and site (right panel) information of different ILC2 sub-clusters indicated by colors. e Dot plots show the top 10 DEGs in Pre_ILC2, Kit_ILC2, CCR9_ILC2, CRTH2_ILC2, PTGS2_ILC2 clusters. Colors represent the average expression and size encodes the proportion of gene-expressing cells. f ILC-C2 cluster in human fetal thymus identified previously (GEO: GSE133341) is projected to UMAP of ILC2 sub-clusters. ILC-C2 cells are highlighted and the ILC2 cells in this study are in gray (below panel). g Representative flow cytometry results show the frequencies of CCR9_ILC2 in fetal thymus (upper) and intestine (below). CCR9_ILC2 cell is gated as Lin–CD45+CD127+CD117–CRTH2–CCR9+. h Flow cytometry results show the expression of GATA-3 and RORγt in CD117–CRTH2–CCR9+ ILC2 (red), conventional CRTH2+ ILC2 (orange) and CD117+CRTH2– ILC3 (blue) cells.
