Fig. 6: CF501/RBD-Fc provides long-term protection against SARS-CoV-2 challenge in hACE2-Tg mice. | Cell Research

Fig. 6: CF501/RBD-Fc provides long-term protection against SARS-CoV-2 challenge in hACE2-Tg mice.

From: A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice, rabbits and NHPs

Fig. 6

a Immunization and challenge procedure for the hACE2-Tg mice to evaluate immediate protection efficacy. The hACE2-Tg mice were immunized with CF501/RBD-Fc at days 0, 14, and 28, respectively. Control hACE2-Tg mice were treated with an equal volume of PBS. The SARS-CoV-2 (strain: SH01) challenge was conducted on day 38. b SARS-CoV-2 (strain: SH01) challenge was conducted 10 days post-final immunization. Body weight changes were monitored for hACE2-Tg mice in CF501/RBD-Fc and control groups. c–e The SARS-CoV-2 challenge (strain: SH01) was conducted 10 days post-final immunization. SARS-CoV-2 viral loads were determined using RT-qPCR at 4 dpc in lungs (c), brains (d), and intestines (e). Data are shown as mean ± sem. f Immunization and challenge procedures for evaluating the long-term protective efficacy in hACE2-Tg mice. The hACE2-Tg mice were immunized with CF501/RBD-Fc and Alum/RBD-Fc on days 0, 14, and 28, respectively. Control hACE2-Tg mice were treated with an equal volume of PBS. The SARS-CoV-2 (strain: SH01) challenge was conducted 6 months post-1st immunization. g The SARS-CoV-2 (strain: SH01) challenge was conducted 6 months post-1st immunization. Body weight changes were monitored for hACE2-Tg mice in CF501/RBD-Fc, Alum/RBD-Fc, and control groups. h–j The SARS-CoV-2 (strain: SH01) challenge was conducted 6 months post-1st immunization. SARS-CoV-2 viral loads were determined using RT-qPCR at 4 dpc in lungs (h), brains (i), and intestines (g). Data are shown as means ± SEM. k Correlation between the body weight change and viral loads in lungs for mice challenged at 6 months. l–n Cytokines and chemokines, such as IFN-β (l), CXCL-10 (m), and CCL-2 (n), in lungs from mice challenged at 6 months post-1st immunization were determined using RT-qPCR. Data were shown as means ± SEM. o Histopathological staining of lungs from mice challenged at 6 months. Statistical analyses were performed using two-way ANOVA for (b) and (g). Statistical analyses for (c), (d), (e), (h), (i), (j), (l) and (m) were performed using one-way ANOVA. *P < 0.05, **P < 0.001, ***P < 0.0001.

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