Fig. 1: Cryo-EM structures of the IR of the S. cerevisiae NPC.
From: Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex
a, b Cryo-EM density map (a) and the atomic model (b) of the intact IR. The map and model are shown in top view (upper panel, dotted lines indicate the cross section corresponding to the side view) and side view (lower panel). Different colors represent different IR monomers. Threshold contour level is 0.22 σ. The gray blob density in the middle of the IR, most probably is a certain complex of FG-repeats, nuclear transport receptors and cargoes. c Color-coded 3D reconstructions of IR dimer (upper panel) and IR monomer (lower panel) showing local resolutions in different views. Threshold contour levels of dimer and monomer are 0.2 σ and 0.13 σ, respectively. The local resolutions were estimated with cryoSPARC and generated in ChimeraX. d Density maps of IR dimer, IR monomer and IR protomer determined in this study. e Gold standard Fourier shell correlation (FSC) curves for the maps of intact IR, IR dimer, IR monomer and IR protomer.
