Fig. 4: Dynamics of nucleosome organization on motif regions predict TF binding landscapes in mouse pronuclei. | Cell Research

Fig. 4: Dynamics of nucleosome organization on motif regions predict TF binding landscapes in mouse pronuclei.

From: Dynamic nucleosome organization after fertilization reveals regulatory factors for mouse zygotic genome activation

Fig. 4

a Nucleosome profiles around CTCF motifs at each PN stage. b Heatmap showing the k-means clustering (k = 3) of TFs based on NDR scores on motifs at each PN stage. M, male PN; F, female PN; h, hpf. c Boxplots showing the enrichment of ZGA gene promoters on motifs of TFs in different clusters defined in b (***P < 0.001; *P < 0.05). d, e Nucleosome profiles around TSSs of different classes of genes in 8-hpf male PN from KD groups. Genes were classified according to whether motifs of MLX (d) or RFX1 (e) are present in promoters. f Boxplots showing the expression level of ZGA genes in KD embryos (*P < 0.05; **P < 0.01; ***P < 0.001; N.S., P > 0.05). g Stacked bar plots showing the percentage of 1-cell and 2-cell embryos in KD groups at the indicated time points. n = 3 biological replicates with ~50 embryos each. Error bars represent SD.

Back to article page