Fig. 3: TPS cells can generate both embryonic and extraembryonic lineages in vivo.
From: Derivation of totipotent-like stem cells with blastocyst-like structure forming potential
a Representative immunofluorescent analysis of expression of embryonic (OCT4) and extraembryonic ectoderm (EOMES) markers in chimeric derivative cells from single TPS cells in E7.5 conceptuses. The stained sections were from the same analyzed conceptus. Scale bars, 200 μm. anti-TD, immuno-staining of tdTomato protein. Similar images were obtained in at least 3 independent experiments. b Enlarged images showing contribution of single TPS derivatives in the extraembryonic ectoderm region. Scale bar, 50 μm. anti-TD, immuno-staining of tdTomato protein. c Representative images showing contribution of single TPS derivative cells in E10.5 embryo, yolk sac and placenta. Scale bar, 500 μm. Td, endogenous tdTomato. BF, bright field. Similar images were obtained in at least 3 independent experiments. d Representative immunofluorescent analysis of E10.5 chimeric placenta generated by injection of single TPS cells (tdTomato labeled). 2-cell embryo-derived TPS cells were used. The left panels show the original images and the right panels show the enlarged images. anti-TD, immuno-staining of tdTomato protein. Scale bars, 2 mm (left panels) and 20 μm (right panels). Similar images were obtained in at least 3 independent experiments. e UMAP plot showing the 19 main clusters in E17.5 chimeric placenta. Pink dotted line indicates extraembryonic cell lineages. f UMAP plot showing tdTomato expression in E17.5 chimeric placenta. Td+, tdTomato-positive cells. Td–, tdTomato-negative cells. g Heatmap showing the average expression of specific marker genes for each cluster from f.
