Fig. 4: Binding modes of non-peptidic agonists.

a Interactions between L-054,522 and SSTR2. Involved residues of SSTR2 are shown as sticks and colored by protactinium. L-054,522 is shown as sticks and colored by violet. b Schematic representation of the interactions between SSTR2 and L-054,522 analyzed using LigPlot⁺ program.⁵⁹ Atoms are shown as circle and colored by black (carbon), red (oxygen) and blue (nitrogen). Polar interactions are indicated by red dash lines. c Inhibition of forskolin-stimulated cAMP accumulation of WT SSTR2 and SSTR2 mutants induced by L-054,522. Bars represent the differences between the calculated L-054,522 potency (pEC₅₀) of WT and mutants. Data are shown as means ± SEM from at least three independent experiments. d Interactions between J-2156 and SSTR4. Involved residues of SSTR4 are shown as sticks and colored by orange. J-2156 is shown as sticks and colored by sky blue. e Schematic representation of the interactions between SSTR4 and J-2156 analyzed using LigPlot⁺ program. f Inhibition of forskolin-stimulated cAMP accumulation of WT SSTR4 and SSTR4 mutants induced by J-2156. The P value was defined as: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. nd (not determined) indicates that a robust concentration response curve could not be determined within the concentration range tested. Detailed statistical evaluation is shown in Supplementary information, Table S3.