Fig. 1: Overall architecture of hSPCA1.

a Topology diagram of hSPCA1. Conserved cytoplasmic domains and TM helices are schematically illustrated. The domains are colored as follows: A domain, cornflower blue; N domain, forest green; P domain, peru (orange-brown); N-terminal region, red; C-terminal region, deep blue; TM1–TM2, cyan; TM3–TM4, hot pink; TM5–TM10, medium purple. b Cryo-EM map (contoured at 5.3 σ) of hSPCA1 in the CaE1-ATP state, shown with the same color scheme as in a. c Atomic models of the six resolved structures of hSPCA1.