Fig. 3: Ligand-binding pocket of GPR3.
From: Identification of oleic acid as an endogenous ligand of GPR3
a The overall shape and density of 3 ligands of GPR3. Density maps of CHS, PAL and OA are set at contour levels of 3.0–5.0 in PyMol. b Detailed view of the ligand-binding pocket. Density maps of OA (blue mesh) and water molecules (W1 and W2, gray mesh) are set at contour levels of 5.0 and 3.0, respectively, in PyMol. c Analysis of the hydrophobic interaction between receptor and ligand via LigPlot. d cAMP assay of GPR3 mutants under condition without addition of exogenous ligand (endogenous ligand of the medium). Data are presented as means ± SEM; n = 3 independent samples; n.s. not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Two-sided t-test. e OA dose-response curve of stably expressed GPR3 mutants. Data are presented as means ± SEM; n = 3 independent samples. OA was added to the cell culture medium at the designated dose.
