Fig. 6: Structural organization of the reRNA.
From: Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs
a Schematic representation of the reRNA and target DNA. The disordered regions are enclosed in black dashed boxes. The pseudoknot regions are enclosed in red dashed boxes. The nucleotide colors are the same as the cartoon representations in Fig. 5c. TS, target strand; NTS, non-target strand; TAM, target adjacent motif; PK, pseudoknot. b Structure of the reRNA scaffold. c The conformation of PK1 (right panel) and PK2 (left panel) d In vitro cleavage of dsDNA substrates by ISFba1 TnpB RNP with full-length reRNA or truncated or mutant reRNA. AAAA, four consecutive ATP linker used to replace the nucleotides in the mutant regions. The agarose gel was visualized by EB staining. Data shown are representative of three independent experiments.
