Fig. 1: Observation of GABA-containing vesicles and GABA-induced currents in rat AVNPCs. | Cell Research

Fig. 1: Observation of GABA-containing vesicles and GABA-induced currents in rat AVNPCs.

From: A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles

Fig. 1

a Representative TEM image showing abundant transmitter vesicle-like ultrastructures beneath the surface membranes of rat AVNPCs. Right, a magnified version of the white box in the left image. White arrows indicate the vesicles. Scale bar, 500 nm. b Immunofluorescence images showing the colocalization of GABA with the vesicle marker CAST in single rat AVNPCs. Scale bar, 10 μm. c Representative patch clamp recordings of whole-cell currents in rat AVNPCs elicited by various concentrations of GABA (0.01 μM, 0.1 μM, 1 μM, 10 μM, 100 μM). The holding potential was set at –60 mV. Horizontal bar, 500 ms; vertical bar, 10 pA. d Quantification of the GABA-elicited currents amplitude with different concentrations of GABA. n = 8 cells for each column. Data are shown as the means ± SD. P values were calculated by one-way ANOVA with Dunnett’s multiple comparison test.

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