Fig. 2: Identification of the endogenous GABAergic system in AVNPCs. | Cell Research

Fig. 2: Identification of the endogenous GABAergic system in AVNPCs.

From: A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles

Fig. 2

a Illustrator and workflow for the single-cell gene expression analysis of AVNPCs from Hcn4CreERT2(+); Rosa26TomRed+mice. b Heatmap showing GABAergic system gene expression in single AVNPCs from Hcn4CreERT2(+); Rosa26TomRed+ mice. Rows indicate the AVNPC samples, and columns indicate the cycle threshold (Ct) values of GABAergic system genes. Ct values were scaled and the relative gene expression was demonstrated using color scales. A score of –1 (red) indicates a high expression level, and a score of 1 (blue) indicates a low level of expression. The data were obtained from 52 AVNPCs from 5 Hcn4CreERT2(+); Rosa26TomRed+ mice. c Immunofluorescence staining showing the expression and localization of GABA metabolic enzymes (GAD2, GABA-T, and SSADH), GABAA receptors (GABRA3, GABRB2, and GABRG2), GABA transporters (vGAT and GAT-1) in single mouse AVNPCs. Scale bar, 10 μm.

Back to article page