Fig. 7: Knockdown of key genes in GABAergic system alters electrical conduction between the atria and ventricles in vivo. | Cell Research

Fig. 7: Knockdown of key genes in GABAergic system alters electrical conduction between the atria and ventricles in vivo.

From: A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles

Fig. 7

a Top, a cartoon illustration of experimental design. Bottom, schematic diagram of local injection of virus into rat AVN. AO aorta, RA right atrium. b Fluorescence microscopy confirmed that RFP was highly expressed in Koch’s triangle injected with AAV2/9 virus. Bright-field (left), RFP (middle) and merged images (right) of hearts injected with AAV2/9 virus. The yellow triangle indicates the triangle of Koch area. The asterisk denotes AVN location. Scale bar, 2 mm. FO fossa ovalis, TV tricuspid valve, TT tendon of Todaro, CS coronary sinus, IVC inferior vena cava. c Representative ECGs obtained from conscious rats through a telemetric recording system after administration of AAV2/9-Control, AAV2/9-Gabrb2, AAV2/9-Slc32a1 or AAV2/9-Abat virus. Scale bars are 100 ms (horizontal) and 0.5 mV (vertical). d–h Quantification of the average PR intervals (d), P wave durations (e), QRS durations (f), QT intervals (g) and heart rates (BPM, beats per minute) (h) of rats in the indicated groups. For AAV2/9-Control group, n = 7; for AAV2/9-Gabrb2 group, n = 6; for AAV2/9-Slc32a1 group, n = 6; for AAV2/9-Abat group, n = 6. Data are shown as means ± SD. P values were calculated by one-way ANOVA with Dunnett’s multiple comparison test.

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