Fig. 3: SMYD5 and RPL40 K22me3 promote global translation output by enhancing mRNA TE. | Cell Research

Fig. 3: SMYD5 and RPL40 K22me3 promote global translation output by enhancing mRNA TE.

From: SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma

Fig. 3

a, b WB analyses of newly synthesized proteins in the negative control (NC) and SMYD5-depleted (KO1) Huh7 and SNU449 cell lines by AHA-click labeling (a) and puromycin (b) labeling approaches. The intensity of NC cells was normalized as 1.00 as indicated under the different treatments. c Measurement of newly synthesized proteins in the WT and RPL40 K22R KI 293T cell lines by SUnset/Puromycin labeling approach. d Scatterplots of ribo-seq depicting the changes in transcription level (x-axis) and translation level (y-axis). The red and blue dots represent genes with stable mRNA levels but increase or decrease in RPF levels. FC cutoff is 1.5. e Volcanoplots representing the TE changes from ribo-seq. Genes with FC > 1.5 or < 1/1.5 and Padj < 0.05 were colored. Average TE is between NC and SMYD5 KO1, allowing for an evaluation of whether genes are highly/lowly translated. f Footprint of ribo-seq across normalized CDS regions, normalized by mitochondrial RPF.

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