Fig. 1: Structural insights into ligand recognition and G-protein coupling of GPR55.
From: Structural insight into GPR55 ligand recognition and G-protein coupling
a, b Overall structures of LPI-bound and AM251-bound GPR55/G13 complexes, respectively. Left panel, orthogonal view of the cryo-EM density map; right panel, model of the complex in the same view and color scheme with the left panel. c LPI binding and recognition mode in GPR55. d Hydrophobicity analysis of the LPI-binding pocket in GPR55. e AM251 binding and recognition mode in GPR55. f AM251 only occupies the middle-upper part of the hydrophobic pocket of GPR55. g BRET assays of G13 dissociation for GPR55 ligand-binding pocket mutants. Data are presented as means ± SD; n = 3. h Details of the G13 engagement with GPR55.
