Fig. 4: Spermatid-specific broad H3K4me3 domains are coupled to transcriptional potentials of key genes for spermiogenesis. | Cell Research

Fig. 4: Spermatid-specific broad H3K4me3 domains are coupled to transcriptional potentials of key genes for spermiogenesis.

From: SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development

Fig. 4

a Boxplot showing the expression levels of target genes associated with broad, sharp, and control H3K4me3 peaks at the RS4 stage of mouse spermatogenesis. ***P < 0.001. b Functional enrichment analysis of broad H3K4me3 marked genes in mouse spermatids utilizing GREAT to infer potential functional pathways and associated biological processes. c, d Heatmaps showing the RNA expression levels (c) and broad H3K4me3 intensities (d) of RS broad H3K4me3-marked genes throughout mouse spermatogenesis. Genes are sorted based on the peak expression timing, progressing from early to late stages. e Transcription factor-binding motifs enriched within broad H3K4me3 domains in mouse RSs. f Boxplots showing the expression levels of enhancer RNAs in RSs across three categories: broad H3K4me3 domains that overlap with SEs (BroadSE+), broad H3K4me3 domains without overlapping with SEs (BroadSE–), and typical enhancers (Ens). g Transcription factor-binding motifs enriched within broad H3K4me3 domains in human RSs.

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