Fig. 1: Biochemical characterization of NbaSPARDA complex.
From: Target DNA-induced filament formation and nuclease activation of SPARDA complex
a Genome organization of the NbaSPARDA system, with the domain organization of NbaSPARDA complex color coded. b Size exclusion chromatography profile and cryo-EM images of gRNA-bound NbaSPARDA complex with tDNA of different lengths (upper: 13 nt; lower: 21 nt). The cryo-EM images are labeled with a scale bar or the length indicator. c FQ assay to determine the collateral nuclease activity of NbaSPARDA against ssDNA in the presence of gRNA and tDNA of varying lengths. d FQ assay to determine the collateral nuclease activity of NbaSPARDA against ssDNA in the presence of gRNA and tDNA containing 2-nt mismatches. FQ assay was performed in triplicate, and the error bars represent the standard deviations.
