Fig. 2: Structure of guide-bound NbaSPARDA complex.
From: Target DNA-induced filament formation and nuclease activation of SPARDA complex
a Size exclusion chromatography profile and 2D average images of apo and guide-bound NbaSPARDA complexes (upper: apo; lower: guide-bound). b Overall structure of guide-bound NbaSPARDA complex from two views. c Interaction details between APAZ-DREN protein and NbaAgo protein within the NbaSPARDA complex. d Interaction details at the APAZ–APAZ interface. e Interaction details at the Ago–Ago interface. f Interaction details between gRNA and NbaAgo domain. g FQ assay to determine the collateral nuclease activity of wild-type and mutant NbaSPARDA against ssDNA. FQ assay was performed in triplicate, and the error bars represent the standard deviations.
