Fig. 1: A collection of migrasomes.

a TSPAN4-GFP overexpressing MCA-205 cancer cell migrated on FN-coated surface. Scale bar, 5 μm. The enlarged box shows migrasomes and retraction fibers. Scale bar, 2 μm. b Neutrophil cells isolated from mouse bone marrow were activated with 1 μM fMLP and migrated on a confocal dish. Cells were stained with WGA-647 before imaging. Scale bar, 5 μm. The enlarged box shows migrasomes and retraction fibers. Scale bar, 1 μm. c Transmission electron microscopy image of the lowest ultra-thin section (in contact with the culture dish) of a L929 cell. Scale bar, 500 nm. Enlarged migrasome is shown in the down left panels. Scale bar, 500 nm. d Representative transmission electron microscopy images of L929 cell treated with 2 mM CCCP. Scale bar, 20 nm. e Neutrophil cells isolated from mouse bone marrow were activated with 1 μM fMLP and migrated on silicon surfaces and observed by field emission scanning electron microscopy. Scale bar, 2 μm. Boxed regions in the right down is an enlarged migrasome. Scale bar, 200 nm. f Intravital imaging of neutrophils in mouse liver. Neutrophils were labeled with PE-anti-Ly6G6C (green), and blood vessels were labeled with AF647-WGA (purple). The white arrow indicates the migrasome. Scale bar, 2 μm. g Intravital imaging of mouse circulating monocytes and monocyte-derived extracellular particles. LPS (12 mg/kg) was injected into mice by intraperitoneal injection. Monocytes were labeled with CCR2-PE antibody (yellow), and blood vessels were labeled with AF647-WGA (cyan). Scale bar, 5 μm. h Confocal image of migrasomes in zebrafish. A single blastomere of an embryo at the eight-cell stage was injected with tspan4a-GFP mRNA. Spinning disk microscopy was used to acquire 3D images of gastrula cells (6 hpf). i Transmission electron microscopy image of CAM of chicken embryos from day 9. Individual migrasomes are pointed by black arrows. Scale bar, 1 μm.