Fig. 3: The SH3RF2/CaMKII/PPP1CC protein complex.
From: Autism-related proteins form a complex to maintain the striatal asymmetry in mice
a Co-IP assay indicated that SH3RF2 can interact with CaMKII and PPP1CC. Short: short exposure; Long: long exposure. b Immunostaining of cultured striatal neurons expressing 3× HA-SH3RF2 (transfected at 15 div, fixed at 21 div) using antibodies against HA, PPP1CC, and Thr287/286-phosphorylated CaMKII (p-CaMKII). Arrows: spines with co-localization of SH3RF2 with PPP1CC (left panel) and with p-CaMKII (right panel). Spines marked with yellow arrows were magnified. Scale bars, 5 μm. c Co-IP assay and quantitative result showed reduced interaction between CaMKII and PPP1CC in the striatum of Sh3rf2 KO mice. n = 4 mice per group. One-sample t-tests compared normalized mean of KO against the theoretical value of 1 (representing WT). d, e Western blot and quantitative results displayed the expression levels of total CaMKII and p-CaMKII in the left and right striatum. n = 5 mice per group. Linear mixed model (genotype × hemisphere + (1|batch)) with ANOVA and Tukey post-hoc tests. f Model of the role of SH3RF2/CaMKII/PPP1CC protein complex in synapses. All data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ****P < 0.0001; ns no significance.
