Fig. 4: SH3RF2/CaMKII/PPP1CC complex regulates the asymmetric expression of GluR1 on PSD.
From: Autism-related proteins form a complex to maintain the striatal asymmetry in mice
a Representative western blot results demonstrated the expression levels of AMPAR and NMDAR subunits in the PSD fraction of left and right striatum. SYN (synaptophysin) serves as a negative control. b–g Quantitative results displayed the expression levels of AMPAR and NMDAR subunits in the PSD fraction. n = 4 mice per group. Linear mixed model (genotype × hemisphere + (1|batch)) with ANOVA and Tukey post-hoc tests. h, i Western blot and quantitative results displayed the protein levels of total GluR1 and phosphorylated GluR1 in the left and right striatum. n = 3 mice per group. Two-way ANOVA with Sidak’s multiple comparisons test. j Molecular mechanism by which SH3RF2 deficiency leads to impaired functional lateralization of striatal MSNs. All data are presented as mean ± SEM; *P < 0.05; ****P < 0.0001; ns no significance.
