Fig. 1
From: Vitamin C promotes the proliferation and effector functions of human γδ T cells
Distinct effects of VC and pVC on the in vitro expansion of Vγ9Vδ2 T cells. a PBMCs obtained from four healthy donors were stimulated with HMBPP (left panel) or ZOL (right panel) in the presence of IL-2. VC or pVC was added at the indicated concentrations. The number of viable Vγ9 T cells per microculture well was determined in triplicate by flow cytometry after 7 days of culture. Each symbol indicates an individual healthy donor. Horizontal bars represent the mean values. b PBMCs from three healthy donors were stimulated with BrHPP or HMBPP and IL-2 in the absence or presence of 50 μg/mL (173 μM) pVC. The number of viable Vγ9 T cells per microculture well was determined in triplicate by flow cytometry after 7 days of culture. Each symbol indicates an individual healthy donor. Horizontal bars represent the mean values. c Purified γδ T cells (50,000/well) from the same donors as in (b) were stimulated with BrHPP or HMBPP and IL-2 in the absence or presence of 50 μg/mL (173 μM) pVC. The number of viable Vγ9 T cells per microculture well was determined in triplicate by flow cytometry after 7 days of culture. Each symbol indicates an individual healthy donor. Horizontal bars represent the mean values. Statistical significance was calculated with the paired, two-tailed Student’s t-test. ns, not significant
