Fig. 6
From: Vitamin C promotes the proliferation and effector functions of human γδ T cells
Modulation of surface markers, transcription factor expression, and cytokine secretion by pVC. a, b Twelve-day-expanded γδ T-cell lines were restimulated with BrHPP in the presence or absence of 50 μg/mL (173 μM) pVC. Four and six days after restimulation, cell surface expression of a CD80 (clone L307.4) and b CD86 (clone 2331[FUN-1]) was measured by flow cytometry. Summary bar graphs of four independent experiments (mean ± SD) showing the frequency of positive cells among gated Vδ2 T cells. c, d γδ T cells freshly purified from PBMCs were stimulated with BrHPP in IL-2-containing medium in the presence or absence of pVC. At day 8, cells were harvested, stained intracellularly for GATA-3 (clone L50-823) and T-bet (clone 4B10) (black) and their corresponding isotype controls (gray). c Representative dot plots of one out of six independent experiments are shown. d Summary bar graphs represent the mean values ± SD of six independent experiments. e The indicated cytokines present in the supernatants of the cell cultures in (c, d) were measured by LEGENDplexTM bead-based array assay (n = 3). f, g Quantification of IFN-γ (n = 6) and IL-13 (n = 9) by ELISA in the supernatants obtained on day 8 after initial stimulation. Statistical significance was calculated with the paired, two-tailed Student’s t-test. *p < 0.05 and **p < 0.01
