Fig. 1
From: IL-29 promoted obesity-induced inflammation and insulin resistance
Higher serum IL-29 levels in obese patients. a, b Level of IL-29 in serum from 10 healthy individuals, 13 overweight patients (pre-obese), 15 class I obese patients, 15 class II obese patients, and 11 class III obese patients were analyzed using ELISA. Data are expressed as mean ± SD of triplicate samples from a single experiment and are representative of three independent experiments. *P < 0.05 compared with healthy individuals. c The expression of IL-29 and its receptors (IL-28R1 and IL-10R2) in visceral adipose tissues isolated from obese patients was detected and analyzed using RT-PCR with specific primers. β-actin was an internal control. d Immunofluorescence staining for the adipocyte markers FABP-4 (red), IL-29 (green), and DAPI (blue) in the visceral adipose tissue of obese patients. Colocalization of IL-29 with FABP-4 is shown in yellow in the merged image. Scale bars = 30 μm. e Immunofluorescence staining for the macrophage markers CD68 (red), IL-29 (green), and DAPI (blue) in the visceral adipose tissue of obese patients. Colocalization of IL-29 with CD68 is shown in yellow in the merged image. Scale bars = 30 μm. f The expression of IL-29 and its receptors in visceral adipocytes isolated from obese patients was detected using FACS. Data are representative of three independent experiments
