Fig. 4

Inhibition of IL-29 reduced IL-6, IL-8, and MCP-1 production in mature adipocyte-LPS-stimulated macrophage coculture system. a Schematic representation of in vitro inflamed adipose tissue model. b The expression of IL-29 and its receptors in monocytes, macrophages, and LPS-induced macrophages. c The level of IL-29 in coculture system was measured using ELISA. *P < 0.05 compared with SGBS only group. d Levels of IL-6, IL-8, MCP-1, and IL-29 in coculture system were detected using ELISA after anti-IL-29 mAb (1.5 μg/ml) or control mIgG (1.5 μg/ml) treatment for 48 h. *P < 0.05, **P < 0.01 compared with the mIgG-treated group. e After coculture for 48 h, the total RNA from SGBS cells was isolated for RT-qPCR with primers specific for IL-1β, IL-6, IL-8, and MCP-1 to amplify the transcripts. β-actin was an internal control. *P < 0.05, **P < 0.01 compared with the mIgG-treated group. Data are expressed as mean ± SEM and are representative of three independent experiments