Fig. 4 | Cellular & Molecular Immunology

Fig. 4

From: BCL-6 suppresses miR-142-3p/5p expression in SLE CD4+ T cells by modulating histone methylation and acetylation of the miR-142 promoter

Fig. 4

BCL-6 regulates histone H3K27me3 and H3K9/K14ac levels in the miR-142 promoter. a, b ChIP-qPCR analysis of the enrichment of histone H3K27me3 (a) and H3K9/K14ac (b) in the miR-142 promoter in chromatin fractions extracted from normal CD4+ T cells transfected with BCL-6 expression plasmid or negative control plasmid. The results are presented relative to those obtained with input DNA prepared from untreated chromatin. Data represent the mean of three independent experiments. c, d ChIP-qPCR analysis of the enrichment of histone H3K27me3 (c) and H3K9/K14ac (d) in the miR-142 promoter in chromatin fractions extracted from normal (n = 12) and SLE (n = 12) CD4+ T cells. The results are presented relative to those obtained with input DNA prepared from untreated chromatin. e, f ChIP-qPCR analysis of the enrichment of histone H3K27me3 (e) and H3K9/K14ac (f) in the miR-142 promoter in chromatin fractions extracted from SLE CD4+ T cells transfected with BCL-6 interference plasmid or negative control plasmid. The results are presented relative to those obtained with input DNA prepared from untreated chromatin. Data represent the mean of three independent experiments (*P < 0.05, **P < 0.01)

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