Fig. 3
From: TCR–pMHC bond conformation controls TCR ligand discrimination
The Effects of TCR microclusters and CD4 binding on TCR–pMHC bond conformation. smFRET TCR–pMHC bond distance measurements in the presence of the actin polymerization inhibitor latrunculin A (LA) (1 µM) (a) or 10 µg/mL anti-CD4 blocking antibody (b) at 37 °C. In each case, cells were pretreated with LA or antibody for 1 h before introduction to the planar bilayer. Histograms of TCR–pMHC bond distances for the K5, MCC, and 102S pMHCs are shown. Each histogram used 339–1295 single TCR–pMHC bond trajectories to identify the most probable distance for each pMHC. c The TCR–pMHC bond distances for each pMHC in different conditions measured by FRET. Data are presented as the most probable distance ± standard deviation (SD)
