Fig. 1

Zfp281 expression is induced by TCR stimulation in CD4⁺ T cells and does not affect T-cell development. a q-PCR detection of the Zfp281 expression levels in different T-cell subsets, including DN (Lin⁻CD4⁻CD8⁻), DP (CD4⁺CD8⁺), CD4 SP (TCRβ⁺CD4⁺CD8⁻), CD8 SP (TCRβ⁺CD4⁻CD8⁺) thymocytes and splenic CD4⁺, CD8⁺ T cells (n = 3). b Immunoblot detection of the Zfp281 protein in sorted CD4⁺, CD8⁺ splenic T cells. q-PCR (c) and immunoblot analysis (d) of Zfp281 in purified CD4⁺ splenic T cells stimulated with anti-CD3 and anti-CD28 mAbs for the indicated times. The results are presented relative to Actb expression (n = 3). N.S, non-specific band. e Flow cytometric analysis and quantification of the DN, DP, CD4 SP, and CD8 SP thymocyte subpopulations from Zfp281^fl/fl (WT) and Zfp281^fl/flCd4^Cre (cKO) mice (n = 5). f Flow cytometric analysis of CD4 and CD8 expression in splenocytes from the indicated mice, and quantitation of the absolute cell numbers (n = 5). Data are presented as the mean ± s.d. A two-tailed, unpaired t-test was used. NS not significant. *P < 0.05, **P < 0.01, ***P < 0.001