Fig. 5 | Cellular & Molecular Immunology

Fig. 5

From: The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription

Fig. 5

Zfp281-deficient CD4+ T cells show decreased expression of T-cell activation-related genes. a Scatter plot of the expression of all genes in WT and Zfp281-deficient naive CD4+ T cells stimulated with the anti-CD3 mAb for 4 h in the presence of APC detected by RNA-Seq analysis. Downregulated genes are indicated in blue, upregulated genes are indicated in red. b Gene ontology (GO) analysis of the differentially expressed genes as shown in a. Blue, downregulated genes; red, upregulated genes. c Heat map comparing the indicated mRNA transcripts. d q-PCR analysis of the Ctla-4 transcript levels in naive CD4+ T cells from WT and cKO mice unstimulated or stimulated with coated anti-CD3 (5 μg/mL) and soluble anti-CD28 (3 μg/mL) for 4 h. e CTLA-4 expression in Tconv cells with or without plate-bound anti-CD3 stimulation for 8 h. f Detection of CTLA-4 in Tconv cells from mice with the OT-II background treated with or without the OVA peptide for 8 h. Data are representative of three independent experiments (mean ± s.d.). A two-tailed, unpaired t-test was used. NS not significant. *P < 0.05, **P < 0.01

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