Fig. 2

CD4⁺ T cells from Themis^-/- mice have defective effector functions in vivo. Naïve sorted CD4⁺ T cells from Themis^-/- and Themis^+/+ mice were adoptively transferred into Rag1^-/- hosts. A Percent change in body weight of Rag1^-/- hosts after adoptive transfer. Error bars represent SEMs. Colon (B) length and (C) weight/length ratio of Rag1^-/- hosts at 6 weeks after adoptive transfer. Absolute numbers of (D) total T cells, (E) total CD4⁺, (G) IFNγ⁺CD4⁺Foxp3^- and (H) IL-17α⁺CD4⁺Foxp3^- T cells in the peripheral and mesenteric lymph nodes of Rag1^-/- hosts at 6 weeks after adoptive transfer. Data are representative of two individual experiments with n = 3–4 biological replicates per group per experiment. *p < 0.5, **p < 0.01, ***p < 0.001 as determined by Student’s t test. All error bars represent SDs except those in 2(a), which represent the standard error mean