Table 1 List of antibodies used for flow cytometry, immunoprecipitation and western blotting
Antigen | Host | Target | Fluorophore | Clone | Company | Catalog no |
|---|---|---|---|---|---|---|
IFNγ | Rat | Mouse | PE | XMG1.2 | eBiosciences | 12-7311-82 |
TNFα | Rat | Mouse | APC | MP6-XT22 | eBiosciences | 17-7321-82 |
IL-2 | Rat | Mouse | PE-Cy7 | JES6-5H4 | BioLegend | 503832 |
CD4 | Rat | Mouse | BV421 | RM4-5 | BioLegend | 100544 |
CD4 | Rat | Mouse | V450 | RM4-5 | eBiosciences | 48-0042-82 |
CD4 | Rat | Mouse | AF 488 | RM4-5 | BD Biosciences | 557667 |
CD4 | Rat | Mouse | PE | RM4-5 | BioLegend | 100512 |
CD4 | Rat | Mouse | APC | RM4-5 | eBiosciences | 17-0042-83 |
CD4 | Rat | Mouse | APC | GK1.5 | eBiosciences | 17-0041-83 |
CD8 | Rat | Mouse | BUV395 | 53-6.7 | BD Biosciences | 563786 |
CD8 | Rat | Mouse | APC | 53-6.7 | eBiosciences | 17-0081-83 |
CD25 | Rat | Mouse | AF 488 | 7D4 | eBiosciences | 53-0252-82 |
CD25 | Rat | Mouse | PE | 7D4 | BD Biosciences | 558642 |
CD25 | Rat | Mouse | PE-Cy7 | PC61 | BioLegend | 102016 |
TCRβ | Rat | Mouse | BV510 | H57-597 | BD Biosciences | 563221 |
CD44 | Rat | Mouse | BV711 | IM7 | BD Biosciences | 563971 |
Mouse IgG | Goat | Rabbit | AF647 | Polyclonal | Invitrogen | A-21245 |
CD3e | Armenian Hamster | Mouse | Purified | 145-2C11 | eBiosciences | 14-0031-85 |
CD28 | Syrian Hamster | Mouse | Purified | 37.51 | eBiosciences | 16-0281-86 |
IR (CD220) | Mouse | Mouse | Purified | 46/CD220 | BD Biosciences | 610109 |
Themis | Rabbit | Mouse | Purified | Polyclonal | Merck | 06–1328 |
Grb2 | Mouse | Mouse | Purified | 81/GRB2 | BD Biosciences | 610112 |
GAPDH | Rabbit | Mouse | Purified | 14C10 | Cell Signaling | 2118 |
Rabbit IgG | Goat | Rabbit | 680LT | Polyclonal | LI-COR Biosciences | 926–68021 |
Mouse IgG | Goat | Mouse | 800CW | Polyclonal | LI-COR Biosciences | 926–32210 |
c-Myc | Rabbit | Mouse | Purified | D3N8F | Cell Signaling | 13987S |
pS6 (S235/236) | Rabbit | Mouse | Purified | D57.2.2E | Cell Signaling | 4858S |
