Fig. 2

CD146⁺ T cells and macrophages are increased during eCM development. a Immunohistochemical staining of CD45 during eCM development. Scale bar, 50 µm. b Immunofluorescent staining of CD146 and CD45, as well as CD4 or CD8, in the brains of eCM mice. c The percentages of CD4⁺ , CD8⁺ , and CD19⁺ cells in the MDSC (CD11b⁻Gr-1⁻) populations isolated from the spleen (SP) and peripheral blood (PB) of healthy or eCM mice (n = 10). d The percentages of CD146⁺ CD4 and CD8 T cells in the SP and PB of healthy and eCM mice (n = 10). e Representative flow cytometric analysis of brain infiltrated leukocytes. f The percentages of CD146⁺ CD4 and CD8 T cells, as well as CD146⁺ macrophages, in the brains of healthy and eCM mice (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001. The sata are representative of at least three independent experiments