Fig. 3
From: SARS-CoV-2 nsp12 attenuates type I interferon production by inhibiting IRF3 nuclear translocation
SARS-CoV-2 nsp12 does not inhibit the phosphorylation of IRF3. HEK293T cells were transfected with a control plasmid or nsp12 expression plasmid. After 24 h, the cells were infected with SeV and collected at the indicated times. Whole-cell lysates were analyzed by western blotting for p-IRF3, IRF3, TBK1, nsp12, and P protein of SeV. b HEK293T cells were transfected with a control plasmid or increasing amounts of plasmids expressing nsp12. After 24 h, the cells were infected with SeV for 8 h. Whole-cell lysates were analyzed by western blotting for p-IRF3, IRF3, nsp12, and P protein of SeV
