Fig. 6
From: SARS-CoV-2 nsp12 attenuates type I interferon production by inhibiting IRF3 nuclear translocation
IFN regulation by SARS-CoV-2 nsp12 is not related to the NiRAN domain. a Schematic diagram of SARS-CoV-2 nsp12. b Effects of SARS-CoV-2 nsp12 and the ΔNiRAN mutant on SeV-induced IFN-β promoter activation. HEK293T cells were transfected with an IFN-β reporter plasmid along with a control plasmid or plasmids expressing wild-type nsp12 or the ΔNiRAN mutant. The cells were infected with SeV for 12 h and assayed for luciferase activity. The expression levels of the indicated protein were analyzed by western blotting. The two-tailed unpaired t-test was used for two-group comparisons compared to the vector group, ***P < 0.001
