Fig. 1
From: Generation of HIV-resistant cells with a single-domain antibody: implications for HIV-1 gene therapy
Cell-surface expression of GPI-anchored antibodies in transduced TZM-bl cells. A Diagram of the lentiviral transfer vector expressing GPI-anchored antibodies. The encoding sequence of m36.4 or FluIgG03 scFv was genetically linked with sequences encoding a His tag and the GPI attachment signal of DAF. RRE, Rev response element; cPPT, central polypurine track; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. B Expression of GPI-m36.4 or GPI-FluIgG03 on the surface of transduced TZM-bl cells with or without PI-PLC treatment, which was detected by an anti-His tag antibody and analyzed by FACS analysis. C Localization of GPI-m36.4 or GPI-FluIgG03 in transduced TZM-bl cells with confocal analysis. Alexa555-CtxB, cells stained with an Alexa Fluor 555-conjugated cholera toxin B subunit; Alexa488-Anti-His: cells stained with a mouse anti-His tag antibody followed by an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody
