Fig. 3
From: Generation of HIV-resistant cells with a single-domain antibody: implications for HIV-1 gene therapy
Inhibitory activity of GPI-anchored antibodies during cell–cell HIV-1 transmission. A Infection with the replication-competent CCR5-tropic HIV-1 isolates RHPA.c/2635 (subtype B), THRO.c/2626 (subtype B), and MJ4 (subtype C) in TZM-bl cells depends on the presence of DEAE-dextran when the isolates are in the form of cell-free viruses. RLU, relative luciferase units; T/F, transmitted/founder virus. The assay was performed in triplicate and repeated two times, and representative data are shown. B Infection with cell-associated RHPA.c/2635, HRO.c/2626, and MJ4 in TZM-bl cells was independent of DEAE-dextran during cell-cell transmission. The assay was performed in triplicate and repeated two times, and representative data are shown. C Inhibition of cell-cell HIV-1 transmission by GPI-anchored antibodies. TZM-bl cells expressing GPI-m36.4 or GPI-FluIgG03 were used as targets and cocultured with CEMss-CCR5 donor cells that were preinfected with one of the three viruses. % cell-cell transmission was monitored by quantifying the production of the luciferase reporter in the TZM-bl cells. Error bars represent the means ± standard deviations (SD) of three independent experiments with triplicate samples
