Fig. 1 | Cellular & Molecular Immunology

Fig. 1

From: The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13

Fig. 1

OTUB1 expression is upregulated in DCs by T. gondii infection and LPS stimulation. a, b Mice were infected i.p. with five T. gondii cysts (a) or challenged with LPS (b) for the indicated time periods. CD11c+ cells were isolated from spleens by magnetic sorting, and OTUB1 expression was measured by WB analysis (upper panels). The lower panels show the relative expression of OTUB1 (n = 3 for each group). c–h GM-CSF-expanded (c, e, and g) and FLT3L-expanded (d, f, and h) BMDCs generated from C57BL/6 mice were stimulated in vitro with tachyzoites at an MOI of 3 (c, d), 1 μg/ml TgPFN (e, f), or 500 ng/ml LPS (g, h) for the indicated time periods. OTUB1 expression in BMDCs was analyzed by WB analysis (upper panels). The lower panels show the relative expression of OTUB1 (n = 3 for each group). Data are displayed as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001

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