Fig. 4

OTUB1 deletion in DC impairs the cytokine response during T. gondii infection. a–c OTUB1^fl/fl and CD11c-Cre OTUB1^fl/fl mice were infected i.p. with 50,000 tachyzoites for 12 and 48 h, respectively. Peritoneal cells were collected by lavage of the peritoneal cavity and analyzed by flow cytometry to detect IL-12 production in CD11c⁺ CD8a⁺, CD11c⁺ PDCA-1⁺, and CD11c⁺ CD11b⁺ DCs (a); UBC13, MHC II, CD40, CD86 and CD80 expression in CD11c⁺ CD8a⁺, CD11c⁺ PDCA-1⁺, and CD11c⁺ CD11b⁺ DCs (b); and IFN-γ production in NK cells (c). Representative flow cytometry plots (left panels) and statistics (right panels) are shown (n = 8). Data are shown as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001; n.s. not significant