Cellular & Molecular Immunology

Table 1 Pseudogene usage in GC events occurred at VH of L15H15 strains treated with TSA for 42 days

From: Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line

HC	CDR1						CDR2						CDR3
HC	A12-3	A12-4	A12-5	B7-3	B7-9	B7-11	A12-3	A12-4	A12-5	B7-3	B7-9	B7-11	A12-3	A12-4	A12-5	B7-3	B7-9	B7-11
Total reads^a	717	662	1176	981	994	1174	717	662	1176	981	994	1174	717	662	1176	981	994	1174
V	603	586	991	779	850	994	580	559	904	748	855	945	691	628	1119	894	946	1108
Full match^b	64	35	101	111	51	99	6	2	25	16	5	23	0	3	9	6	10	5
pVH001	0	0	0	2	0	1	0	0	0	0	0	0	0	0	0	0	0	0
pVH003	1	0	0	1	1	3	0	0	0	1	0	0	0	0	0	0	0	0
pVH005	0	1	0	0	1	3	0	0	0	1	0	0	0	0	2	0	0	0
pVH007	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
pVH009	3	4	7	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0
pVH011	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
pVH013	11	3	14	15	11	9	0	0	2	2	0	5	0	0	0	1	0	0
pVH015	25	14	57	29	14	33	0	1	1	2	0	3	0	0	0	0	0	0
pVH017	8	4	6	10	5	9	0	0	0	0	0	1	0	3	5	3	9	4
pVH019	0	1	2	1	0	0	0	1	2	0	1	0	0	0	0	1	0	1
pVH021	3	0	3	3	4	20	0	0	0	0	0	5	0	0	0	0	0	0
pVH023	12	6	10	49	13	18	6	0	19	10	3	8	0	0	0	0	0	0
pVH025	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0
pVH027	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
pVH029	1	1	1	0	1	2	0	0	1	0	1	1	0	0	0	0	1	0
Partial match^c	16	11	18	47	23	26	19	12	34	25	19	33	7	9	14	7	5	18

^aThe total number of observed sequences in each library
^bThe number of sequences in which whole pseudogene sequences are copied
^cThe number of sequences not identical to any of initial V sequence or pseudogenes

Back to article page

Search

Advanced search

Quick links