Fig. 3
From: Dysregulated immunity in PID patients with low GARP expression on Tregs due to mutations in LRRC32
Phenotype of Garp-deficient mice. A GARP and LAP levels on the surface of Tregs were assessed by flow cytometry. Representative staining histograms after stimulation with anti-CD3 and anti-CD28 antibodies for 2 days are shown. B The Foxp3 level was assessed by intracellular flow cytometry. Representative staining histograms and data for one representative experiment out of four independent experiments with five mice per group are shown. Horizontal lines indicate mean values. MFI mean fluorescence intensity. C Counts of T cell populations in spleens from 12 control and 12 Garp-deficient 12-week-old mice. Horizontal lines indicate mean values. D Lungs, livers, and intestines from 10- to 12-week-old control and Garp-deficient mice were immunohistochemically stained for CD3. Representative sections are shown
