Fig. 6
From: Dysregulated immunity in PID patients with low GARP expression on Tregs due to mutations in LRRC32
Analysis of Hdac9 expression and Foxp3 acetylation. Heat maps of statistically significant differentially expressed genes involved in A apoptosis or B cell cycle regulation, C characteristic of the Treg phenotype, or D encoding cytokines in Garp-deficient and control Tregs. E Heat maps of genes encoding histone acetyltransferases and deacetylases. F, G Expression of CD25, NRP1, CTLA4, and IL-10. The expression of CD25, NRP1, and CTLA4 was measured in freshly isolated Tregs by intracellular flow cytometry after gating for Foxp3 (F). The expression of IL-10 was measured in freshly isolated Tregs by intracellular flow cytometry after stimulation with PMA and ionomycin (G). Representative staining histograms and a summary of one representative experiment out of two independent experiments with five mice per group are depicted. Horizontal lines indicate mean values. MFI mean fluorescence intensity. H mRNA levels of the indicated histone deacetylases in relation to the Actb mRNA level were determined by real-time PCR analysis. The summarized data of four independent experiments performed in triplicate are shown as the mean ± SD. I Analysis of Foxp3 acetylation. Representative staining results with a FRET antibody pair specific for Foxp3 and AcK in control and Garp-deficient CD4+ T cells and a summary of the results from one representative experiment out of four independent experiments with five mice per group. Horizontal lines indicate mean values. AcK acetylated lysin
