Fig. 1

Loss of CD147 expression destabilizes Tregs in the periphery. A Comparison of Foxp3+ Treg numbers in the thymus, lymph nodes, spleen, and circulating blood of WT and CD147^T-KO mice by flow cytometry. B–I For the inflammatory bowel disease (IBD) model, 2.5 × 10⁴ CD45.2 + CD4 + CD25 + Tregs were sorted from the spleens of CD147^T-KO mice (n = 7) or their WT littermate controls (n = 8) and then mixed with 5 × 10⁵ CD4 + CD25 − conventional T cells from WT CD45.1 + B6 mice. Cell mixtures were intraperitoneally transferred into Rag1−/− mice. Recipient mice were euthanized 44 days later, and colon and spleen tissues were isolated for histopathological analysis. B Weight changes in recipient mice after adoptive transfer were normalized to their initial body weights before transfer. C Representative H&E stained images of colon tissues. D Summary of histopathological scores. E Absolute numbers of splenocytes. F Absolute numbers of CD4 + CD45.1+ cells in spleens. G CD45.1 + T cells from spleens were stimulated with PdBU and ionomycin for 4 h and then stained for the intracellular cytokines IFNγ and IL17a. H Absolute numbers of CD4 + CD45.2+ cells in spleens. I Percentages of Foxp3+ cells among CD45.2+ cells and mean fluorescence intensity (MFI) of Foxp3 staining in Foxp3+ Tregs