Fig. 3

Attenuation of arthritis via regulation of the phenotype of immune cells in p40-EBI3 transgenic C57BL/6 mice achieved by microinjection of the transgene. Collagen-induced arthritis (CIA) was induced in WT or p40-EBI3 transgenic C57BL/6 mice as described in the Methods section. A Representative results from one of three independent experiments are shown for the clinical scores (left) and incidences of arthritis (right) for each treatment group over time. B The concentrations of total IgG in sera from mice in each group at 12 weeks after CII immunization were determined by ELISA. C Spleens were obtained from mice in each group at 12 weeks after CII immunization and analyzed by flow cytometry for B220⁺GL-7⁺ germinal center B cells and B220⁻CD138⁺ plasma cells. Representative flow cytometry plots from one of three independent experiments are shown (left); the results are depicted as the mean ± SD of three mice per group (right). D Mice were sacrificed at 12 weeks after CIA induction; the populations of IFN-γ⁺, IL-4⁺, IL-17⁺, and CD25⁺Foxp3⁺ cells among splenic CD4⁺ T cells were analyzed by flow cytometry. Representative flow cytometry plots from one of three independent experiments are shown (left); the results are depicted as the mean ± SD of three mice per group (right) (*p < 0.05, **p < 0.01, ***p < 0.001).