Fig. 1: KLF14 is upregulated in the macrophages of in vivo and in vitro murine sepsis models.

A–D Peripheral mononuclear macrophages (PBMCs), lung tissues, colon tissues and spleen tissues were isolated from endotoxemic mice (n = 3; LPS 20 mg/kg ip, 12 h), septic mice (n = 3; CLP 25-gauge needle, 12 h) and healthy controls (n = 3). A, C The expression of the KLF14 protein in PBMCs from endotoxemic, septic, and healthy mice. B, D The mRNA expression of KLF14 in PBMCs and lung, colon, and spleen tissues from endotoxemic, septic, and healthy mice. The data are presented as the mean ± SD. n = 3, **P < 0.01, ***P < 0.001. E–J RAW264.7 macrophages, THP-1-derived macrophages, and BMDMs were treated for 0 h, 2 h, 6 h, 12 h, and 24 h with LPS (100 ng/ml). Protein (E–G) and mRNA (H–J) expression of KLF14 in RAW264.7 macrophages, THP-1-derived macrophages, and BMDMs. The data are presented as the mean ± SD. n = 3, **P < 0.01, ***P < 0.001. K Immunofluorescence staining of the macrophage markers F4/80 (red) and KLF14 (green) and DAPI (blue) in lung sections taken 12 h after intraperitoneal LPS injection or cecal ligation puncture. Scale bar, 20 µm. The data are presented as the mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001