Fig. 3: KLF14 plays a crucial role in the immune response of macrophages during sepsis. | Cellular & Molecular Immunology

Fig. 3: KLF14 plays a crucial role in the immune response of macrophages during sepsis.

From: The transcription factor KLF14 regulates macrophage glycolysis and immune function by inhibiting HK2 in sepsis

Fig. 3

A Western blot analysis of the indicated protein expression in BMDMs confirmed the deletion of KLF14. B The protein expression of IL-1β and IL-6 in BMDMs after stimulation with LPS (100 ng/ml) for 6 h. C Heatmap of inflammatory cytokine changes in WT and KLF14−/− BMDMs. D ELISA analysis of cytokines secreted from BMDMs after LPS (100 ng/ml) stimulation for 6 h. E Western blot analysis of the indicated protein expression in RAW264.7 macrophages confirmed the overexpression of KLF14. The indicated protein expression (F), mRNA expression (G), and ELISA results (H) of inflammatory cytokines in the control group and KLF14 overexpression group after LPS (100 ng/ml) stimulation for 6 h were assayed. The data are presented as the mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001. I The lactate production, J phagocytosis, and K glucose uptake of WT and KLF14−/− BMDMs stimulated with or without 100 ng/ml LPS for 6 h were assayed. The data are presented as the mean ± SD. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page