Fig. 5 | Cellular & Molecular Immunology

Fig. 5

From: SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages

Fig. 5

AO-MPs facilitate viral clearance by decreasing the lysosomal pH a AMs were pretreated with AO-MPs for 30 min and then stained with LysoSensor™ Green DND-189 for 30 min at 37 °C. The cells were observed under a confocal microscope. b The same as (a), except that cells were stained with LysoSensor™ Yellow/Blue DND-160. The pH value was detected by a microplate reader. c Cells were processed to separate the lysosomal and cytoplasmic fractions of AO-MP-treated or untreated RAW264.7 cells. The purified lysosomes were detected by western blotting. d The lysates of purified lysosomes isolated from AO-MP-treated (MP-lyso) or untreated (Lyso) RAW264.7 cells were incubated with SARS-CoV-2 for 30 min at 37 °C and then used to infect Vero E6 cells for 48 h. Cells were stained with an anti-NP antibody. Lyso, lysosomes. Scale bar, 50 μm. The data represent the mean ± SD of three independent experiments. * p < 0.05, *** p < 0.001; two-tailed Student’s t-test (a, b) or one-way ANOVA (d)

Back to article page