Fig. 2
ESCC TAMs constitutively express CCL22. A The differential expression of cytokines in TAMs versus PBMs from the same ESCC patient, as evaluated by a cytokine antibody array assay. B The secretion of the indicated cytokines from MDMs, pol-TAMs, and pri-TAMs, as evaluated by ELISA. (C) Representative IHC images of the expression of CCL22 in normal esophageal tissues or ESCC tissues (LN+). D Percentages of ESCC patients with high expression of stromal CCL22 and low expression of stromal CCL22 according to different clinical parameters, as follows: tumor stage, tumor status and lymph node status. E Representative sequential IHC images of the expression of CD204 and CCL22 in ESCC tissues. Stromal CCL22 expression was positively associated with CD204 expression in 117 primary ESCC specimens. F Kaplan‒Meier curves of overall survival for ESCC patients stratified by low vs. high numbers of CCL22-positive TAMs. Original magnification, ×5, ×10 or ×20
