Fig. 3
From: Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2
Substitution of S982A and L18F weakens the generation of binding antibodies. The area under the curve (AUC) of anti-spike (A) and anti-RBD (B) IgG induced by the 19 designed groups of DNA immunogens (n = 3 for each group). The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8. Data were analyzed for statistical significance using the unpaired Student’s t test. Data are shown as the mean with SD in each group. Dotted black lines indicate the limit of quantification (LOQ). *P < 0.05; **P < 0.01; ***P < 0.001
